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KMID : 0379520100260020101
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한국독성학회지
2010 Volume.26 No. 2 p.101 ~ p.108
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Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice
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Lee Sang-Kyu
Lee Dong-Ju
Jeon Tae-Won
Ko Gyu-Sub
Yoo Se-Hyun
Ha Hyun-Woo
Kang Mi-Jeong
Kang Won-Ku
Kim Sang-Kyum
Jeong Tae-Cheon
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Abstract
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Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 ㎎/㎏ in corn oil once for dose response or treated orally with 1-BB at 1500 ㎎/㎏ for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.
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KEYWORD
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1-Bromobutane, Glutathione, Conjugation, Immunotoxicity, In vivo
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